Pseudomonas aeruginosa PAO1, PA0336 (rppH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0336
Name
rppH
Synonym: ygdP
Replicon chromosome
Genomic location 378096 - 378575 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249027.1
GI 15595533
Affymetrix PA0336_at
Entrez 882290
GenBank AAG03725.1
INSDC AAG03725.1
NCBI Locus Tag PA0336
protein_id(GenBank) gb|AAG03725.1|AE004471_8|gnl|PseudoCAP|PA0336
TIGR NTL03PA00337
UniParc UPI0000130902
UniProtKB Acc Q9X4P2
UniProtKB ID RPPH_PSEAE
UniRef100 UniRef100_A6UYD9
UniRef50 UniRef50_A6UYD9
UniRef90 UniRef90_A6UYD9

Product

Feature Type CDS
Coding Frame 1
Product Name
RNA pyrophosphohydrolase, RppH
Product Name Confidence: Class 1
Synonyms dinucleoside oligophosphate pyrophosphatase
Nudix hydrolase YgdP
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.17
Kyte-Doolittle Hydrophobicity Value -0.500
Molecular Weight (kDa) 18.7
Isoelectric Point (pI) 9.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 247 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000328 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0336
Search term: rppH

Human Homologs

References

The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A).
Bessman MJ, Walsh JD, Dunn CA, Swaminathan J, Weldon JE, Shen J
J Biol Chem 2001 Oct 12;276(41):37834-8
PubMed ID: 11479323
Nudix-type RNA pyrophosphohydrolase provides homeostasis of virulence factor pyocyanin and functions as a global regulator in Pseudomonas aeruginosa.
Kujawa M, Lirski M, Ziecina M, Drabinska J, Modzelan M, Kraszewska E
Mol Microbiol 2017 Nov;106(3):381-394
PubMed ID: 28833678