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Pseudomonas Genome Database - Annotation for PA4756
               gene PA4756                
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Key to gene image


View in GBrowse: (?)
45 putative orthologs Acknowledgements for this annotation
Feature Overview
Strain Pseudomonas aeruginosa PAO1 (Tax ID: 208964) Replicon Pseudomonas aeruginosa PAO1 chromosome, complete genome.
(5688 genes, 6264404 bp) [ RefSeq ]
Feature type gene  Genomic location 5339864 - 5343085 (- strand)
Name carB  (View this annotation at our new website) Alternate Names
Locus tag PA4756 (View 7 updates) Submit an Update Tranposon Mutant Available? 1 mutant(s) available.
Product Information
Feature type CDS
Product Name carbamoylphosphate synthetase large subunit
Product Name Confidence Class 1: Function experimentally demonstrated in this species.
Alternate Names
Charge at pH 7 (Predicted):-33.47
Kyte-Doolittle Hydropathy Score (Predicted):-0.096
Molecular Weight (Predicted):117.3 kDa
Isoelectric Point (Predicted):4.78
Evidence of Translation:Detected by MALDI-TOF/TOF (PMID:19333994).
Evidence of Translation:Detected using LC-MS/MS (117.33 kDa, PMID:21751344)
Subcellular localization
CompartmentTypeConfidence LevelExplanation of Localization Confidence
Cytoplasmic Primary Class 3: Subcellular localization computationally predicted by PSORTb V3.0.
Outer Membrane Vesicle Secondary Class 1: Subcellular localization experimentally demonstrated in Pseudomonas aeruginosa.
Orthologs/Comparative Genomics
Pseudomonas Ortholog Group Explanation of Orthologous Groups
Number of Orthologs in Pseudomonas species 45 putative orthologs
OrtholugeDB (New!) Explanation of Orthologous Groups
Secondary Genomic Features contained in PA4756
Name Sequence Start Stop Strand PubMed IDs Cross Reference Other Notes
promoter region upstream of greA
Retrieve 5339867 5340053 + 9139926
RegTransBase62014
Note: Manually curated from experimental data. Annotation transferred to all sequenced strains of species.
ISphoA/hah insertion site
5341178 5341178 - 14617778
UWGC: 51016
ISphoA/hah insertion site
5341321 5341321 + 14617778
UWGC: 48411
Inverted repeat (palindrome) of 5341490..5341481
Retrieve 5341387 5341396 + 10827456
Software: Palindrome (Emboss 6.2.0)
mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
5341464 5341464 N/A 15805499
PseudoCAP: PAO1_lux_73_F9
Inverted repeat (palindrome) of 5341387..5341396
Retrieve 5341481 5341490 - 10827456
Software: Palindrome (Emboss 6.2.0)
mini-Tn5 lux transposon insertion site (Transcriptional fusion)
5341670 5341670 N/A 15805499
PseudoCAP: PAO1_lux_75_D3
Alternate Annotations and Cross-References
GI 15599950
Precomputed BLAST results NCBI BLINK
RefSeq NP_253444
TIGR NTL03PA04757
UniProtKB Acc P38100
UniProtKB ID CARB_PSEAE
Affymetrix PA4756_carB_at
KEGG PA4756
GBrowse Click GBrowse for a GBrowse View of this and other annotations.
PseudoCyc Click here to view metabolic pathways in PseudoCyc.
Gene Ontology (GO) - Participate in the 2014 Gene Ontology Annotation initiative and get your name on a consortium publication!
GO Accession GO Term Evidence
Code
Reviewed
by curator?
Reference
Molecular Function
GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IDA Yes
Biological Process
GO:0071230 cellular response to amino acid stimulus IDA Yes
GO:0006536 glutamate metabolic process IDA Yes
GO:0009220 pyrimidine ribonucleotide biosynthetic process IEA No
Cellular Component
Function Classification
PseudoCAP Function Class Nucleotide biosynthesis and metabolism
Amino acid biosynthesis and metabolism
Pathways
Pathways Explanation of Pathways Field
Name Database Xref
Pyrimidine metabolism PseudoCAP
Arginine and proline metabolism PseudoCAP
Glutamate metabolism PseudoCAP
arginine biosynthesis PseudoCyc ARGSYNBSUB-PWY
de novo biosynthesis of pyrimidine ribonucleotides PseudoCyc PWY0-162
his+purine+pyrimidine biosynthesis PseudoCyc PRPP-PWY
Pyrimidine metabolism KEGG pae00240 Computationally Generated
Alanine, aspartate and glutamate metabolism KEGG pae00250 Computationally Generated
Metabolic pathways KEGG pae01100 Computationally Generated
Reactions
EC Number 6.3.5.5
Homology
BLINK Precomputed BLAST results at BLINK
Most Similar Human Homolog
ENSP00000233072, Percent Identity: 40.75%, E-Value: 0.0, Ensembl Release: 73.
Description: Carbamoyl-phosphate synthase [ammonia], mitochondrial Precursor (EC 6.3.4.16)(Carbamoyl-phosphate synthetase I)(CPSase I) [Source:UniProtKB/Swiss-Prot;Acc:P31327]
Structure Features
CDD predictions Explanation of CDD predictions
CDD Accession External Accession Database Short Name Description Start Stop E-value Release Number
235393 PRK05294 PRK carB carbamoyl phosphate synthase large subunit; Reviewed 1 1068 0.0 3.10
233379 TIGR01369 TIGRFAM CPSaseII_lrg carbamoyl-phosphate synthase, large subunit 2 1053 0.0 3.10
223534 COG0458 COG CarB Carbamoylphosphate synthase large subunit (split gene in MJ) [Amino acid transport and metabolism / Nucleotide transport and metabolism] 13 408 0.0 3.10
190425 pfam02786 Pfam CPSase_L_D2 Carbamoyl-phosphate synthase L chain, ATP binding domain 128 335 1e-72 3.10
198164 smart01096 SMART CPSase_L_D3 Carbamoyl-phosphate synthetase large chain, oligomerisation domain 424 547 2e-54 3.10
238712 cd01424 CD MGS_CPS_II Methylglyoxal synthase-like domain from type II glutamine-dependent carbamoyl phosphate synthetase (CSP) 942 1051 1e-41 3.10
 
Microarray Expression Data
NCBI GEO Link
DNA Sequence
Upstream 500 BP Region TGCTGCTGTCGGCCCTTGGCGTGGCGTCCCTGCTGAAGGCCGAGCCGATGCTGTTCATCGGCCTGAAGTACCTCGGTGCGGCCTACCTGTTCTATCTCGG
CGTCGGCATGCTGCGGGGCGCCTGGCGCAAGCTGCGCAATCCCGAAGCGACGGCGGCGCAGGCCGAACAGGTGGATGTCCACCAGCCGTTCCGCAAGGCG
CTGCTGCTCAGCCTGTCGAATCCCAAGGCGATCCTGTTCTTCATCTCCTTCTTCATCCAGTTCGTCGATCCCGGCTATGCCTATCCGGGGCTGTCGTTCC
TGGTGCTGGCGGTGATCCTGGAACTGGTCAGCGCCCTTTACCTGAGCTTCCTGATTTTCACCGGTGTGCGGCTGGCGGCCTGGTTCCGCCGGCGGCAACG
GTTGGCTGCCGGCGCCACTTCGGGCGTCGGCGCCCTGTTCGTCGGCTTTGGTGTGAAGCTGGCCACCGCAACCCTGTCCTGATTATTGCAACGAGAGTCC
C


BLAST search against this genome or other genomes in database
Need more than 500 BP? Try our sequence retrieval tool or view intergenic regions in GBrowse.

DNA Sequence ATGCCAAAACGTACAGACATAAAGAGCATCCTGATCCTCGGTGCCGGCCCCATCGTGATTGGCCAGGCCTGCGAATTCGACTACTCCGGCGCCCAGGCCT
GCAAGGCCCTGCGCGAGGAGGGCTACCGCGTCATCCTGGTGAACTCCAACCCCGCGACCATCATGACCGATCCGGCGATGGCCGACGCGACCTACATCGA
GCCGATCAAGTGGGCCACCGTGGCCAAGATCATCGAGAAGGAACGCCCCGACGCGCTGCTGCCGACCATGGGCGGCCAGACTGCGTTGAACTGCGCCCTG
GACCTGGAGCGCCACGGCGTGCTGGAGAAGTTCGGCGTGGAGATGATCGGCGCCAATGCCGATACCATCGACAAGGCCGAGGACCGCTCGCGCTTCGACA
AGGCGATGAAGGATATCGGCCTGGCCTGTCCGCGCTCGGGCATCGCCCACAGCATGGAGGAGGCCTACGGCGTGCTCGAGCAGGTCGGCTTCCCCTGCAT
CATCCGTCCGTCCTTCACCATGGGCGGCACCGGCGGCGGTATCGCCTACAACCGTGAAGAGTTCGAAGAGATCTGCGCCCGTGGCCTCGACCTGTCGCCG
ACCAACGAGCTGCTGATCGACGAGTCGCTGATCGGCTGGAAGGAATACGAGATGGAGGTGGTCCGCGACAAGAAGGACAACTGCATCATCGTCTGCTCCA
TCGAGAACTTCGACCCGATGGGCGTGCACACCGGTGACTCGATCACCGTGGCGCCGGCGCAGACCCTGACCGACAAGGAATACCAGATCATGCGCAACGC
CTCGCTGGCGGTATTGCGCGAGATCGGCGTGGAAACCGGCGGTTCCAACGTGCAGTTCGGCATCTGCCCGAACACCGGGCGCATGGTGGTGATCGAGATG
AACCCGCGGGTTTCCCGCTCCTCGGCCCTGGCCTCCAAGGCCACCGGTTTCCCGATCGCCAAGATCGCCGCCAAGCTGGCGGTCGGCTACACCCTCGACG
AACTGCAGAACGACATCACCGGCGGTCGCACCCCGGCGTCGTTCGAGCCGGCCATCGACTACGTGGTGACCAAGATCCCGCGCTTCGCCTTCGAGAAATT
CCCGAAAGCCGATGCCCGCCTGACCACCCAGATGAAGTCGGTCGGCGAAGTCATGGCCATCGGCCGGACCTTCCAGGAGTCGGTGCAGAAAGCCTTGCGT
GGCCTGGAAGTCGGCGCCACCGGCTTCGATCCGAAGCTCGACCTGAACGACCCGGAGGCCGACAGCATCCTCAAGCGCGAGCTGACCGTGCCCAGCGCCG
AGCGTGTCTGGTACGTCGCCGACGCCTTCCGCGCCGGCAAGAGCGTCGAGGAAGTGTTCGAGCTGACCCGCATCGACGAATGGTTCCTGGTGCAGATCGA
GGACCTGGTCAAGGACGAGGAGAAGGTCAAGACCCTCGGCCTGTCCTCCATCGATCGCGAGCTGATGTACAAGCTCAAGCGCAAGGGCTTCTCCGACGCG
CGCCTGGCCAAGCTGCTCGGCGTCACCGAGAAGAACCTGCGCAGCCATCGCCACAAGCTGAAGGTACTGCCGGTCTACAAGCGTGTCGATACCTGCGCCG
CCGAGTTCGCCACCGACACCGCCTACATGTATTCGACCTACGAGGAAGAGTGCGAGGCCAACCCGTCGAGCCGCGAGAAGATCATGATCCTCGGCGGCGG
TCCGAACCGCATCGGCCAGGGCATCGAGTTCGACTACTGCTGCGTGCACGCGGCGCTGGCGATGCGCGAAGACGGCTACGAGACCATCATGGTCAACTGC
AACCCGGAAACCGTCTCCACCGACTATGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGACGTCCTTGAGATCGTCCGCGTCGAGCAGC
CCAAGGGCGTGATCGTCCAGTACGGCGGCCAGACCCCGCTGAAGCTCTGCCGCGCCCTCGAAGAGGCCGGCGTGCCGATCATCGGCACCAGTCCGGACGC
CATCGACCGTGCCGAAGACCGCGAGCGCTTCCAGCAGATGGTCCAGCGCCTGAACCTGCGCCAGCCGGCCAACGCCACCGCGCGCAGCGAAGACGAGGCC
CTGGCCGCGTCCAAGGCCATCGGTTATCCGCTGGTGGTACGCCCGTCCTACGTGCTGGGCGGGCGGGCGATGGAAATCGTCTACCAGGAAGAAGAACTCA
AGCGCTACATGCGTGAGGCGGTTCAGGTTTCCAACGACAGCCCGGTGCTGCTCGACCACTTCCTCAACTGCGCCATCGAAGTCGACATCGACGCGGTATG
CGACGGCGAGATCGTGGTGATCGGCGCGATCATGCAGCATATCGAGCAGGCTGGCGTTCACTCCGGCGACTCCGCCTGTTCGCTGCCGCCGTACTCGCTG
CCGCAGCACATCCAGGACGAGATCCGCGAGCAGGTCAAGAAAATGGCCCTGGAGCTTGGCGTGGTCGGCCTGATGAACGTGCAGATGGCGGTGCAGGGCG
AGGATATCTTCGTCATCGAGGTCAATCCGCGCGCCTCGCGTACCGTGCCGTTCGTCTCCAAGTGCGTCGGCGAGTCGCTGGCCAAGGTCGCCGCCCGCGT
CATGGCCGGCAAGACCCTGGCCGAGGTCGGCTTCACCCAGGAAATCATCCCGCCGTTCTTCAGCGTCAAGGAAGCGGTGTTCCCGTTCGCCAAGTTCCCC
GGCGTCGATCCGATCCTCGGCCCGGAAATGAAGTCCACTGGTGAAGTGATGGGCGTCGGTGATAGCTTTGCCGAAGCCTTTGCCAAGGCTCAGTTGGGGG
CCAGCGAGATTCTCCCGACCGCCGGTTGCGCCTTCATCAGCGTCCGCGAAGACGACAAGCCGTTCGCCGCCCAGGTGGCTGGCGACCTGGTCGCGCTGGG
CTTCGAAGTGGTAGCCACCGCCGGTACCGCGCGTGTCATCGAGGCCGCGGGCCTGCCGGTGCGTCGCGTGAACAAGGTGACCGAAGGTCGCCCGCACGTG
GTGGACATGATCAAGAACGACGAGGTCACCCTGATCATCAACACCACCGAGGGCCGGCAGTCCATCGCTGACTCCTACTCCATTCGTCGAAACGCCCTGC
AGCACAAGATCTGCTGCACCACCACCATTGCGGGTGGGCAGGCGATCTGTGAGGCGCTCAAGTTCGGTCCCGAGAAGACCGTTCGGCGTCTGCAGGATCT
CCACGCAGGAATCAAGGCATGA


BLAST search against this genome or other genomes in database

Downstream 500 BP region GCAAGTTCCCCATGACCGTCCAGGGCGCCCGCGCCCTGGAAGAAGAAGTGAAACACCTGAAGGGCGTGCTGCGCCCGCAGATCAGCCAGGCCATCGCCGA
AGCGCGTGAACTGGGCGACCTCAAGGAAAACGCCGAGTACCATGCGGCGCGCGAACAGCAGGGCATGGTCGAGGCGCGTATCCGCGACATCGAGGCCAAG
CTGTCCAACGCCCAGGTCATCGATGTCACCGCCATCCCGCACAGCGGCAAGGTGATCTTCGGCACCACCGTCGACATCGCCAATGTCGAGACCGACGAGA
CCGTGACCTACCAGATCGTCGGCGACGACGAGGCGGATATCAAGGGTGGCAAGATCTCGGTGAATTCGCCGATCGCCCGTGCGCTGATCGGCAAGACCGA
AGGCGATGCCGTGCTGGTGAGAACCCCGGGCGGCGACGTCGAGTACGAGATCGTCGAGGTTCGCCATATCTAAGCGCGCCCCGTTGCGCGCAGGCGCGAT
C


Protein Sequence
Protein Sequence MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPAMADATYIEPIKWATVAKIIEKERPDALLPTMGGQTALNCAL
DLERHGVLEKFGVEMIGANADTIDKAEDRSRFDKAMKDIGLACPRSGIAHSMEEAYGVLEQVGFPCIIRPSFTMGGTGGGIAYNREEFEEICARGLDLSP
TNELLIDESLIGWKEYEMEVVRDKKDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIGVETGGSNVQFGICPNTGRMVVIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELQNDITGGRTPASFEPAIDYVVTKIPRFAFEKFPKADARLTTQMKSVGEVMAIGRTFQESVQKALR
GLEVGATGFDPKLDLNDPEADSILKRELTVPSAERVWYVADAFRAGKSVEEVFELTRIDEWFLVQIEDLVKDEEKVKTLGLSSIDRELMYKLKRKGFSDA
RLAKLLGVTEKNLRSHRHKLKVLPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSSREKIMILGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLEIVRVEQPKGVIVQYGGQTPLKLCRALEEAGVPIIGTSPDAIDRAEDRERFQQMVQRLNLRQPANATARSEDEA
LAASKAIGYPLVVRPSYVLGGRAMEIVYQEEELKRYMREAVQVSNDSPVLLDHFLNCAIEVDIDAVCDGEIVVIGAIMQHIEQAGVHSGDSACSLPPYSL
PQHIQDEIREQVKKMALELGVVGLMNVQMAVQGEDIFVIEVNPRASRTVPFVSKCVGESLAKVAARVMAGKTLAEVGFTQEIIPPFFSVKEAVFPFAKFP
GVDPILGPEMKSTGEVMGVGDSFAEAFAKAQLGASEILPTAGCAFISVREDDKPFAAQVAGDLVALGFEVVATAGTARVIEAAGLPVRRVNKVTEGRPHV
VDMIKNDEVTLIINTTEGRQSIADSYSIRRNALQHKICCTTTIAGGQAICEALKFGPEKTVRRLQDLHAGIKA


BLAST search against this genome or other genomes in database
Precomputed BLAST results at BLINK
Operons/Regulons
1) carA-PA4757-carB-greA-PA4754 ( View in GBrowse  )
Genomic Location: From 5338973 to 5344903 bp on - strand
Genes:
PA4754 (PA4754) PA4755 (greA) PA4756 (carB) PA4757 (PA4757) PA4758 (carA)
Estimated Length: 5930 bp (From 5338973 to 5344903 bp)
Evidence:
Class 3: Computationally-predicted.
 [PubMed:18988623]
Cross Reference: DOOR (No external cross-reference is available).  
2) carA-PA4757-carB operon ( View in GBrowse  )
Genomic Location: From 5339864 to 5345023 bp on - strand
Genes:
PA4756 (carB) PA4757 (PA4757) PA4758 (carA)
Estimated Length: 5159 bp (From 5339864 to 5345023 bp)
Evidence:
Class 1: Transcription unit experimentally validated in a strain of the same species. The start coordinate will often be based on an experimentally validated transcription start site. For protein-coding genes, unless otherwise specified, the stop will be based on the stop codon.
 [PubMed:8169201]
Cross Reference: PseudoCAP: 41779
Method lacZ fusion experiment, S1 nuclease mapping
References
PubMed Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PMID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS.
Proteomics. 2011 Aug;11(16):3424-9.
PMID: 21751344
Carbamoylphosphate synthetase from Pseudomonas aeruginosa. Subunit composition, kinetic analysis and regulation.
Abdelal AT, Bussey L, Vickers L.
Eur J Biochem. 1983 Jan 1;129(3):697-702.
PMID: 6402363
Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.
Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT.
J Bacteriol. 1994 May;176(9):2532-42.
PMID: 8169201