Pseudomonas aeruginosa PAO1, PA0036 (trpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0036
Name
trpB
Replicon chromosome
Genomic location 37890 - 39098 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248726.1
GI 15595234
Affymetrix PA0036_trpB_at
Entrez 879212
GenBank AAG03426.1
INSDC AAG03426.1
NCBI Locus Tag PA0036
protein_id(GenBank) gb|AAG03426.1|AE004443_4|gnl|PseudoCAP|PA0036
TIGR NTL03PA00037
UniParc UPI0000137520
UniProtKB Acc P07345
UniProtKB ID TRPB_PSEAE
UniRef100 UniRef100_P07345
UniRef50 UniRef50_P07345
UniRef90 UniRef90_P07345

Product

Feature Type CDS
Coding Frame 1
Product Name
tryptophan synthase beta chain
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.08
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 43.7
Isoelectric Point (pI) 6.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 612 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000036 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0036
Search term: trpB

Human Homologs

References

PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.
Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS
Bioinformatics 2010 Jul 1;26(13):1608-15
PubMed ID: 20472543
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994