Pseudomonas aeruginosa PAO1, PA0065

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0065
Name
Replicon chromosome
Genomic location 77432 - 78097 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248755.1
GI 15595263
Affymetrix PA0065_at
Entrez 878495
GenBank AAG03455.1
INSDC AAG03455.1
NCBI Locus Tag PA0065
protein_id(GenBank) gb|AAG03455.1|AE004446_3|gnl|PseudoCAP|PA0065
TIGR NTL03PA00066
UniParc UPI00000C4F12
UniProtKB Acc Q9I767
UniProtKB ID 5NTD_PSEAE
UniRef100 UniRef100_Q9I767
UniRef50 UniRef50_Q9I767
UniRef90 UniRef90_Q9I767

Product

Feature Type CDS
Coding Frame 1
Product Name
haloacid dehalogenase (HAD) family hydrolase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.24
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 25.0
Isoelectric Point (pI) 6.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 406 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000064 (497 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0065

Human Homologs

References

pruR and PA0065 Genes Are Responsible for Decreasing Antibiotic Tolerance by Autoinducer Analog-1 (AIA-1) in Pseudomonas aeruginosa.
Pahlevi MR, Murakami K, Hiroshima Y, Murakami A, Fujii H
Antibiotics (Basel) 2022 Jun 6;11(6)
PubMed ID: 35740179