Pseudomonas aeruginosa PAO1, PA0067 (prlC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0067
Name
prlC
Synonym: opdA
Replicon chromosome
Genomic location 78710 - 80755 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248757.1
GI 15595265
Affymetrix PA0067_prlC_at
Entrez 878482
GenBank AAG03457.1
INSDC AAG03457.1
NCBI Locus Tag PA0067
protein_id(GenBank) gb|AAG03457.1|AE004446_5|gnl|PseudoCAP|PA0067
TIGR NTL03PA00068
UniParc UPI00000C4F14
UniProtKB Acc Q9I765
UniProtKB ID Q9I765_PSEAE
UniRef100 UniRef100_Q9I765
UniRef50 UniRef50_G8PY41
UniRef90 UniRef90_Q9I765

Product

Feature Type CDS
Coding Frame 1
Product Name
oligopeptidase A
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -24.80
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 76.0
Isoelectric Point (pI) 4.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 335 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000066 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0067
Search term: prlC
Search term: oligopeptidase A

Human Homologs

Ensembl 110, assembly GRCh38.p14
neurolysin [Source:HGNC Symbol;Acc:HGNC:16058]
E-value: 9.8e-75
Percent Identity: 28.7

References

Escherichia coli prlC encodes an endopeptidase and is homologous to the Salmonella typhimurium opdA gene.
Conlin CA, Trun NJ, Silhavy TJ, Miller CG
J Bacteriol 1992 Sep;174(18):5881-7
PubMed ID: 1325967
Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle.
Jiang X, Zhang M, Ding Y, Yao J, Chen H, Zhu D, Muramatu M
J Biochem 1998 Nov;124(5):980-5
PubMed ID: 9792922
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994