Pseudomonas aeruginosa PAO1, PA0073 (tagT1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0073
Name
tagT1
Replicon chromosome
Genomic location 86284 - 87003 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_248763.1
GI 15595271
Affymetrix PA0073_at
DNASU PaCD00006032
Entrez 878396
GenBank AAG03463.1
INSDC AAG03463.1
NCBI Locus Tag PA0073
protein_id(GenBank) gb|AAG03463.1|AE004446_11|gnl|PseudoCAP|PA0073
TIGR NTL03PA00074
UniParc UPI00000C4F1A
UniProtKB Acc Q9I759
UniProtKB ID Q9I759_PSEAE
UniRef100 UniRef100_W0YKU8
UniRef50 UniRef50_F6AF48
UniRef90 UniRef90_A6UXK5

Product

Feature Type CDS
Coding Frame 1
Product Name
TagT1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.05
Kyte-Doolittle Hydrophobicity Value -0.101
Molecular Weight (kDa) 26.0
Isoelectric Point (pI) 8.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database Victors : 4220
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575
Source 2
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000072 (411 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0073
Search term: tagT1
Search term: TagT1

Human Homologs

Ensembl 110, assembly GRCh38.p14
ATP binding cassette subfamily B member 4 [Source:HGNC Symbol;Acc:HGNC:45]
E-value: 9.8e-14
Percent Identity: 31.6

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433