Pseudomonas aeruginosa PAO1, PA0158 (triC)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Synonym: triC
Replicon chromosome
Genomic location 179522 - 182569 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs


RefSeq NP_248848.1
GI 15595356
Affymetrix PA0158_at
Entrez 882140
GenBank AAG03548.1
INSDC AAG03548.1
NCBI Locus Tag PA0158
protein_id(GenBank) gb|AAG03548.1|AE004453_11|gnl|PseudoCAP|PA0158
UniParc UPI00000C4F6D
UniProtKB Acc Q9I6X4
UniRef100 UniRef100_U8MJ39
UniRef50 UniRef50_F6A9K9
UniRef90 UniRef90_W0YJL0


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux transporter, TriC
Product Name Confidence: Class 1
Synonyms Resistance-Nodulation-Cell Division (RND) triclosan efflux transporter, TriC efflux transporter)
probable RND efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.33
Kyte-Doolittle Hydrophobicity Value 0.184
Molecular Weight (kDa) 111.9
Isoelectric Point (pI) 5.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
TriC TriC is a resistance nodulation cell division (RND) transporter that is a part of TriABC-OpmH, a triclosan-specific efflux protein. [PMID:17720796] ARO:3003681 protein homolog model Perfect 1938.7 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6VEJ TRANSPORT PROTEIN 01/02/20 TriABC transporter from Pseudomonas aeruginosa Pseudomonas aeruginosa 4.5 ELECTRON MICROSCOPY 100.0

Virulence Evidence

Source 1
Database Victors : 4263
Category Not available / Not known
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Rattus norvegicus
Infection model Rat agar bead model of chronic lung infection
Reference 12023080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000156 (999 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0158
Search term: triC

Human Homologs


Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP
J Bacteriol 2007 Nov;189(21):7600-9
PubMed ID: 17720796