Pseudomonas aeruginosa PAO1, PA0208 (mdcA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0208
Name
mdcA
Replicon chromosome
Genomic location 237232 - 238896 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248899.1
GI 15595405
Affymetrix PA0208_mdcA_at
Entrez 879322
GenBank AAG03597.1
INSDC AAG03597.1
NCBI Locus Tag PA0208
protein_id(GenBank) gb|AAG03597.1|AE004459_1|gnl|PseudoCAP|PA0208
TIGR NTL03PA00209
UniParc UPI00000C4F98
UniProtKB Acc Q9I6T0
UniProtKB ID Q9I6T0_PSEAE
UniRef100 UniRef100_Q9I6T0
UniRef50 UniRef50_O06924
UniRef90 UniRef90_Q9I6T0

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate decarboxylase alpha subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -5.21
Kyte-Doolittle Hydrophobicity Value -0.182
Molecular Weight (kDa) 61.4
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VIT TRANSFERASE 04/17/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with malonate Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.203 X-RAY DIFFRACTION 100.0
5VJ1 TRANSFERASE 04/18/17 Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with coenzyme A Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) 2.995 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 59 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000207 (477 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0208
Search term: mdcA

Human Homologs

References

Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
Hoenke S, Schmid M, Dimroth P
Eur J Biochem 1997 Jun 1;246(2):530-8
PubMed ID: 9208947