Pseudomonas aeruginosa PAO1, PA3542 (alg44)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3542
Name
alg44
Replicon chromosome
Genomic location 3965842 - 3967011 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252232.1
GI 15598738
Affymetrix PA3542_at
Entrez 878770
GenBank AAG06930.1
INSDC AAW77927.1
NCBI Locus Tag PA3542
protein_id(GenBank) gb|AAG06930.1|AE004775_1|gnl|PseudoCAP|PA3542
TIGR NTL03PA03542
UniParc UPI00000C5A60
UniProtKB Acc Q9HY69
UniProtKB ID ALG44_PSEAE
UniRef100 UniRef100_Q9HY69
UniRef50 UniRef50_Q9HY69
UniRef90 UniRef90_Q9HY69

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate biosynthesis protein Alg44
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 5.63
Kyte-Doolittle Hydrophobicity Value -0.067
Molecular Weight (kDa) 41.8
Isoelectric Point (pI) 9.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4RT0 PROTEIN BINDING 11/12/14 Structure of the Alg44 PilZ domain from Pseudomonas aeruginosa PAO1 in complex with c-di-GMP Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 99.1
4RT1 PROTEIN BINDING 11/12/14 Structure of the Alg44 PilZ domain (R95A mutant) from Pseudomonas aeruginosa PAO1 in complex with c-di-GMP Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 98.2
4XRN UNKNOWN FUNCTION 01/21/15 Pilz domain with c-di-gmp of a protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 99.1

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 5 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002124 (491 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3542
Search term: alg44

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Alg44, a unique protein required for alginate biosynthesis in Pseudomonas aeruginosa.
Remminghorst U, Rehm BH
FEBS Lett 2006 Jul 10;580(16):3883-8
PubMed ID: 16797016
Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa.
Maharaj R, May TB, Wang SK, Chakrabarty AM
Gene 1993 Dec 22;136(1-2):267-9
PubMed ID: 8294014