Pseudomonas aeruginosa PAO1, PA3545 (algG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3545
Name
algG
Replicon chromosome
Genomic location 3969942 - 3971573 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252235.1
GI 15598741
Affymetrix PA3545_algG_at
Entrez 879839
GenBank AAG06933.1
INSDC AAG06933.1
NCBI Locus Tag PA3545
protein_id(GenBank) gb|AAG06933.1|AE004775_4|gnl|PseudoCAP|PA3545
TIGR NTL03PA03545
UniParc UPI00000D4273
UniProtKB Acc Q51371
UniProtKB ID ALGG_PSEAE
UniRef100 UniRef100_Q51371
UniRef50 UniRef50_P59828
UniRef90 UniRef90_Q51371

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate-c5-mannuronan-epimerase AlgG
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 7.15
Kyte-Doolittle Hydrophobicity Value -0.314
Molecular Weight (kDa) 59.8
Isoelectric Point (pI) 9.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002121 (490 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3545
Search term: algG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE
J Bacteriol 1994 Apr;176(7):1821-30
PubMed ID: 8144447
Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX.
Monday SR, Schiller NL
J Bacteriol 1996 Feb;178(3):625-32
PubMed ID: 8550492
A new Azotobacter vinelandii mannuronan C-5-epimerase gene (algG) is part of an alg gene cluster physically organized in a manner similar to that in Pseudomonas aeruginosa.
Rehm BH, Ertesvåg H, Valla S
J Bacteriol 1996 Oct;178(20):5884-9
PubMed ID: 8830682