Pseudomonas aeruginosa PAO1, PA3547 (algL)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3547
Name	algL
Replicon	chromosome
Genomic location	3973014 - 3974117 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	AlgL is required for cell viability and maintaining membrane integrity during alginate production
Comment	AlgL prevents accumulation of alginate within the periplasmic space

Cross-References

RefSeq	NP_252237.1
GI	15598743
Affymetrix	PA3547_algL_at
Entrez	878552
GenBank	AAG06935.1
INSDC	AAG06935.1
NCBI Locus Tag	PA3547
protein_id(GenBank)	gb|AAG06935.1|AE004775_6|gnl|PseudoCAP|PA3547
TIGR	NTL03PA03547
UniParc	UPI0000125852
UniProtKB Acc	Q06749
UniProtKB ID	ALGL_PSEAE
UniRef100	UniRef100_Q02R18
UniRef50	UniRef50_Q9L7P2
UniRef90	UniRef90_Q02R18

Product

Feature Type	CDS
Coding Frame	1
Product Name	poly(beta-d-mannuronate) lyase precursor AlgL Product Name Confidence: Class 1
Synonyms	Alginate lyase (AlgL)
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	5.33
Kyte-Doolittle Hydrophobicity Value	-0.449
Molecular Weight (kDa)	40.8
Isoelectric Point (pI)	8.79

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543 Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4OZW	LYASE	02/19/14	Crystal Structure of the periplasmic alginate lyase AlgL H202A mutant	Pseudomonas aeruginosa	1.64	X-RAY DIFFRACTION	99.7
7SA8	LYASE	09/22/21	Crystal Structure of the periplasmic lyase AlgL K66A Mutant	Pseudomonas aeruginosa	2.5	X-RAY DIFFRACTION	99.7
4OZV	LYASE	02/19/14	Crystal Structure of the periplasmic alginate lyase AlgL	Pseudomonas aeruginosa	1.642	X-RAY DIFFRACTION	100.0

Virulence Evidence

Source 1
Database	VFDB : VF0091
Category	Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 12 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002119 (490 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3547 Search term: algL Search term: poly(beta-d-mannuronate) lyase precursor AlgL

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli. Schiller NL, Monday SR, Boyd CM, Keen NT, Ohman DE J Bacteriol 1993 Aug;175(15):4780-9 PubMed ID: 8335634

Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product. Boyd A, Ghosh M, May TB, Shinabarger D, Keogh R, Chakrabarty AM Gene 1993 Sep 6;131(1):1-8 PubMed ID: 8370530

The Pseudomonas aeruginosa homeostasis enzyme AlgL clears the periplasmic space of accumulated alginate during polymer biosynthesis. Gheorghita AA, Wolfram F, Whitfield GB, Jacobs HM, Pfoh R, Wong SSY, Guitor AK, Goodyear MC, Berezuk AM, Khursigara CM, Parsek MR, Howell PL J Biol Chem 2022 Feb;298(2):101560 PubMed ID: 34990713