Pseudomonas aeruginosa PAO1, PA3548 (algI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3548
Name
algI
Replicon chromosome
Genomic location 3974359 - 3975921 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252238.1
GI 15598744
Affymetrix PA3548_algI_at
Entrez 877959
GenBank AAG06936.1
INSDC AAG06936.1
NCBI Locus Tag PA3548
protein_id(GenBank) gb|AAG06936.1|AE004775_7|gnl|PseudoCAP|PA3548
TIGR NTL03PA03548
UniParc UPI00000C5A62
UniProtKB Acc Q51392
UniProtKB ID ALGI_PSEAE
UniRef100 UniRef100_Q51392
UniRef50 UniRef50_Q51392
UniRef90 UniRef90_Q51392

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate o-acetyltransferase AlgI
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.05
Kyte-Doolittle Hydrophobicity Value 0.458
Molecular Weight (kDa) 58.7
Isoelectric Point (pI) 9.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 228 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002118 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3548
Search term: algI

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation.
Franklin MJ, Ohman DE
J Bacteriol 1996 Apr;178(8):2186-95
PubMed ID: 8636017