Pseudomonas aeruginosa PAO1, PA3549 (algJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3549
Name
algJ
Replicon chromosome
Genomic location 3975936 - 3977111 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252239.1
GI 15598745
Affymetrix PA3549_algJ_at
Entrez 878716
GenBank AAG06937.1
INSDC AAG06937.1
NCBI Locus Tag PA3549
protein_id(GenBank) gb|AAG06937.1|AE004775_8|gnl|PseudoCAP|PA3549
TIGR NTL03PA03549
UniParc UPI00000D426A
UniProtKB Acc Q51393
UniProtKB ID ALGJ_PSEAE
UniRef100 UniRef100_Q51393
UniRef50 UniRef50_Q51393
UniRef90 UniRef90_Q51393

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate o-acetyltransferase AlgJ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -9.06
Kyte-Doolittle Hydrophobicity Value -0.363
Molecular Weight (kDa) 43.1
Isoelectric Point (pI) 5.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Periplasmic Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 26 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002117 (498 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3549
Search term: algJ

Human Homologs

References

Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa.
Franklin MJ, Ohman DE
J Bacteriol 2002 Jun;184(11):3000-7
PubMed ID: 12003941
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation.
Franklin MJ, Ohman DE
J Bacteriol 1996 Apr;178(8):2186-95
PubMed ID: 8636017