Pseudomonas aeruginosa PAO1, PA3550 (algF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3550
Name
algF
Replicon chromosome
Genomic location 3977184 - 3977834 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252240.1
GI 15598746
Affymetrix PA3550_algF_at
DNASU PaCD00007557
Entrez 878717
GenBank AAG06938.1
INSDC AAG06938.1
NCBI Locus Tag PA3550
protein_id(GenBank) gb|AAG06938.1|AE004776_1|gnl|PseudoCAP|PA3550
TIGR NTL03PA03550
UniParc UPI00000D421A
UniProtKB Acc Q06062
UniProtKB ID ALGF_PSEAE
UniRef100 UniRef100_Q06062
UniRef50 UniRef50_Q06062
UniRef90 UniRef90_Q06062

Product

Feature Type CDS
Coding Frame 1
Product Name
alginate o-acetyltransferase AlgF
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) 8.40
Kyte-Doolittle Hydrophobicity Value -0.246
Molecular Weight (kDa) 22.8
Isoelectric Point (pI) 9.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6CZT CELL ADHESION 04/09/18 CS-rosetta determined structures of the N-terminal domain of AlgF from P. aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) NOT SOLUTION NMR 100.0
6D10 CELL ADHESION 04/11/18 CS-rosetta determined structures of the C-terminal domain of AlgF from P. aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) NOT SOLUTION NMR, SOLUTION SCATTERING 100.0

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 5 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002116 (487 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3550
Search term: algF

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence and expression of the Pseudomonas aeruginosa algF gene controlling acetylation of alginate.
Shinabarger D, May TB, Boyd A, Ghosh M, Chakrabarty AM
Mol Microbiol 1993 Sep;9(5):1027-35
PubMed ID: 7934909
Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation.
Franklin MJ, Ohman DE
J Bacteriol 1996 Apr;178(8):2186-95
PubMed ID: 8636017
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142