Pseudomonas aeruginosa PAO1, PA3552 (arnB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3552
Name
arnB
Synonym: arnB
Synonym: pmrH
Replicon chromosome
Genomic location 3979860 - 3981008 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_252242.1
GI 15598748
Affymetrix PA3552_at
DNASU PaCD00007558
Entrez 879143
GenBank AAG06940.1
INSDC AAG06940.1
NCBI Locus Tag PA3552
protein_id(GenBank) gb|AAG06940.1|AE004776_3|gnl|PseudoCAP|PA3552
TIGR NTL03PA03552
UniParc UPI00000C5A63
UniProtKB Acc Q9HY65
UniProtKB ID ARNB_PSEAE
UniRef100 UniRef100_Q9HY65
UniRef50 UniRef50_Q7AC24
UniRef90 UniRef90_Q9HY65

Product

Feature Type CDS
Coding Frame 1
Product Name
ArnB
Product Name Confidence: Class 1
Synonyms ArnB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.47
Kyte-Doolittle Hydrophobicity Value -0.061
Molecular Weight (kDa) 41.9
Isoelectric Point (pI) 8.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 551 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002114 (630 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3552
Search term: arnB
Search term: ArnB

Human Homologs

References

Origin of lipid A species modified with 4-amino-4-deoxy-L-arabinose in polymyxin-resistant mutants of Escherichia coli. An aminotransferase (ArnB) that generates UDP-4-deoxyl-L-arabinose.
Breazeale SD, Ribeiro AA, Raetz CR
J. Biol. Chem. 2003 Jul;278(27):24731-9
PubMed ID: 12704196
Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme.
Noland BW, Newman JM, Hendle J, Badger J, Christopher JA, Tresser J, Buchanan MD, Wright TA, Rutter ME, Sanderson WE, Müller-Dieckmann HJ, Gajiwala KS, Buchanan SG
Structure 2002 Nov;10(11):1569-80
PubMed ID: 12429098
Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa.
McPhee JB, Lewenza S, Hancock RE
Mol Microbiol 2003 Oct;50(1):205-17
PubMed ID: 14507375
Deficient Lipid A Remodeling by the arnB Gene Promotes Biofilm Formation in Antimicrobial Peptide Susceptible Pseudomonas aeruginosa.
Segev-Zarko LA, Kapach G, Josten M, Klug YA, Sahl HG, Shai Y
Biochemistry 2018 Apr 3;57(13):2024-2034
PubMed ID: 29518324