Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3764
|
Name |
mltF
Synonym: yfhD |
Replicon | chromosome |
Genomic location | 4219841 - 4221199 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252453.1 |
GI | 15598959 |
Affymetrix | PA3764_at |
Entrez | 880530 |
GenBank | AAG07151.1 |
INSDC | AAG07151.1 |
NCBI Locus Tag | PA3764 |
protein_id(GenBank) | gb|AAG07151.1|AE004795_3|gnl|PseudoCAP|PA3764 |
TIGR | NTL03PA03764 |
UniParc | UPI00006D8743 |
UniProtKB Acc | Q9HXN1 |
UniProtKB ID | MLTF_PSEAE |
UniRef100 | UniRef100_Q9HXN1 |
UniRef50 | UniRef50_Q9HXN1 |
UniRef90 | UniRef90_Q9HXN1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
lytic transglycosylase, MltF
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -4.06 |
Kyte-Doolittle Hydrophobicity Value | -0.608 |
Molecular Weight (kDa) | 51.1 |
Isoelectric Point (pI) | 6.15 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5AA4 | LYASE | 07/23/15 | Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide | PSEUDOMONAS AERUGINOSA | 2.4 | X-RAY DIFFRACTION | 99.8 |
5AA1 | LYASE | 07/23/15 | Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAG-anhNAM-pentapeptide | PSEUDOMONAS AERUGINOSA | 2.89 | X-RAY DIFFRACTION | 99.3 |
5AA2 | LYASE | 07/23/15 | Crystal structure of MltF from Pseudomonas aeruginosa in complex with NAM-pentapeptide. | PSEUDOMONAS AERUGINOSA | 2.8 | X-RAY DIFFRACTION | 99.1 |
5AA3 | SUGAR BINDING PROTEIN | 07/23/15 | Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide | PSEUDOMONAS AERUGINOSA | 3.2 | X-RAY DIFFRACTION | 99.6 |
5A5X | HYDROLASE | 06/23/15 | Crystal Structure of Se-Met MltF from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA PAO1 | 1.801 | X-RAY DIFFRACTION | 99.8 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 244 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001917 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3764
Search term: mltF
Search term: lytic transglycosylase, MltF
|
Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.
Domínguez-Gil T, Lee M, Acebrón-Avalos I, Mahasenan KV, Hesek D, Dik DA, Byun B, Lastochkin E, Fisher JF, Mobashery S, Hermoso JA
Structure 2016 Oct 4;24(10):1729-1741
PubMed ID: 27618662
|