Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3988
|
Name |
lptE
|
Replicon | chromosome |
Genomic location | 4469613 - 4470236 (+ strand) |
RefSeq | NP_252677.1 |
GI | 15599183 |
Affymetrix | PA3988_at |
DNASU | PaCD00007741 |
Entrez | 878911 |
GenBank | AAG07375.1 |
INSDC | AAG07375.1 |
NCBI Locus Tag | PA3988 |
protein_id(GenBank) | gb|AAG07375.1|AE004816_11|gnl|PseudoCAP|PA3988 |
TIGR | NTL03PA03988 |
UniParc | UPI00000C5BB2 |
UniProtKB Acc | Q9HX32 |
UniProtKB ID | Q9HX32_PSEAE |
UniRef100 | UniRef100_W0YY23 |
UniRef50 | UniRef50_S3MKJ9 |
UniRef90 | UniRef90_A6V0C1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
LPS-assembly lipoprotein LptE
|
Synonyms |
hypothetical protein |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.2, molecular weight:22.9, PMID:21674802)
Detected using 2-DE, MALDI-TOF/MS analysis (22.88 kDa, pI 5.2, PMID:16335974)
Detected using LC-MS/MS (22.88 kDa, PMID:21751344)
|
Charge (pH 7) | -4.55 |
Kyte-Doolittle Hydrophobicity Value | -0.448 |
Molecular Weight (kDa) | 22.9 |
Isoelectric Point (pI) | 4.96 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5IVA | TRANSPORT PROTEIN | 03/20/16 | The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex | Pseudomonas aeruginosa | 2.988 | X-RAY DIFFRACTION | 100.0 |
2N8X | LIPID BINDING PROTEIN | 10/28/15 | Solution structure of LptE from Pseudomonas Aerigunosa | Pseudomonas aeruginosa PAO1 | NOT | SOLUTION NMR | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 60 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001693 (534 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3988
Search term: lptE
Search term: LPS-assembly lipoprotein LptE
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Structural basis for lipopolysaccharide insertion in the bacterial outer membrane.
Qiao S, Luo Q, Zhao Y, Zhang XC, Huang Y
Nature 2014 Jul 3;511(7507):108-11
PubMed ID: 24990751
|