Pseudomonas aeruginosa PAO1, PA4563 (rpsT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4563
Name
rpsT
Replicon chromosome
Genomic location 5112663 - 5112938 (+ strand)

Cross-References

RefSeq NP_253253.1
GI 15599759
Affymetrix PA4563_rpsT_at
DNASU PaCD00007976
Entrez 879605
GenBank AAG07951.1
INSDC AAG07951.1
NCBI Locus Tag PA4563
protein_id(GenBank) gb|AAG07951.1|AE004870_2|gnl|PseudoCAP|PA4563
TIGR NTL03PA04564
UniParc UPI0000134E51
UniProtKB Acc Q9HVM1
UniProtKB ID RS20_PSEAE
UniRef100 UniRef100_B7V0A6
UniRef50 UniRef50_Q2KZD7
UniRef90 UniRef90_B7V0A6

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S20
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 15.94
Kyte-Doolittle Hydrophobicity Value -0.516
Molecular Weight (kDa) 9.9
Isoelectric Point (pI) 11.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004222 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4563
Search term: rpsT

Human Homologs

References

Primary structure of protein S20 from the small ribosomal subunit of Escherichia coli.
Wittmann-Liebold B, Marzinzig E, Lehmann A
FEBS Lett 1976 Sep 15;68(1):110-4
PubMed ID: 786731
Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element.
Mackie GA
Nucleic Acids Res 1986 Sep 11;14(17):6965-81
PubMed ID: 2429258
Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20.
Rydén-Aulin M, Shaoping Z, Kylsten P, Isaksson LA
Mol Microbiol 1993 Mar;7(6):983-92
PubMed ID: 7683367
Relationship of the expression of the S20 and L34 ribosomal proteins to polyamine biosynthesis in Escherichia coli.
Panagiotidis CA, Huang SC, Canellakis ES
Int J Biochem Cell Biol 1995 Feb;27(2):157-68
PubMed ID: 7539334