Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5042
|
Name |
pilO
|
Replicon | chromosome |
Genomic location | 5678433 - 5679056 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_253729.1 |
GI | 15600235 |
Affymetrix | PA5042_pilO_at |
DNASU | PaCD00008170 |
Entrez | 880985 |
GenBank | AAG08427.1 |
INSDC | AAG08427.1 |
NCBI Locus Tag | PA5042 |
protein_id(GenBank) | gb|AAG08427.1|AE004917_6|gnl|PseudoCAP|PA5042 |
TIGR | NTL03PA05043 |
UniParc | UPI00000D4260 |
UniProtKB Acc | G3XD51 |
UniProtKB ID | G3XD51_PSEAE |
UniRef100 | UniRef100_W0Z1B7 |
UniRef50 | UniRef50_Q4KJJ2 |
UniRef90 | UniRef90_Q02EX5 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
type 4 fimbrial biogenesis protein PilO
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -6.31 |
Kyte-Doolittle Hydrophobicity Value | -0.004 |
Molecular Weight (kDa) | 22.8 |
Isoelectric Point (pI) | 4.78 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5UVR | MEMBRANE PROTEIN | 02/20/17 | The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.7 | X-RAY DIFFRACTION | 100.0 |
2RJZ | STRUCTURAL GENOMICS, UNKNOWN FUNCTION | 10/16/07 | Crystal structure of the type 4 fimbrial biogenesis protein PilO from Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 99.3 |
Source 1 | |
Database | VFDB : VF0082 |
Category | Type IV pili biosynthesis |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 112 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004645 (941 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5042
Search term: pilO
Search term: type 4 fimbrial biogenesis protein PilO
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Watson AA, McCaul TF, Mattick JS
Mol Microbiol 1995 May;16(3):497-508
PubMed ID: 7565110
|
Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.
Roine E, Nunn DN, Paulin L, Romantschuk M
J Bacteriol 1996 Jan;178(2):410-7
PubMed ID: 8550460
|
PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin.
Ayers M, Sampaleanu LM, Tammam S, Koo J, Harvey H, Howell PL, Burrows LL
J Mol Biol 2009 Nov 20;394(1):128-42
PubMed ID: 19857645
|