Pseudomonas aeruginosa PAO1, PA0157 (triB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0157
Name	triB Synonym: triB
Replicon	chromosome
Genomic location	178455 - 179525 (+ strand)
Transposon Mutants	4 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_248847.1
GI	15595355
Affymetrix	PA0157_at
DNASU	PaCD00006068
Entrez	882134
GenBank	AAG03547.1
INSDC	AAG03547.1
NCBI Locus Tag	PA0157
protein_id(GenBank)	gb|AAG03547.1|AE004453_10|gnl|PseudoCAP|PA0157
TIGR	NTL03PA00158
UniParc	UPI00000C4F6C
UniProtKB Acc	Q9I6X5
UniProtKB ID	Q9I6X5_PSEAE
UniRef100	UniRef100_A0A077JP41
UniRef50	UniRef50_G8PZD9
UniRef90	UniRef90_W0YL24

Product

Feature Type	CDS
Coding Frame	1
Product Name	Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB Product Name Confidence: Class 1
Synonyms	probable RND efflux membrane fusion protein precursor Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB efflux membrane fusion protein precursor)
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	2.22
Kyte-Doolittle Hydrophobicity Value	-0.241
Molecular Weight (kDa)	38.3
Isoelectric Point (pI)	9.12

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
TriB	TriB is a membrane protein that is fused to TriA and both are required for the triclosan efflux pump function of TriABC-OpmH in P. aeruginosa. [PMID:17720796]	ARO:3003680	protein homolog model		Perfect	667.5	100.0	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6VEJ	TRANSPORT PROTEIN	01/02/20	TriABC transporter from Pseudomonas aeruginosa	Pseudomonas aeruginosa	4.5	ELECTRON MICROSCOPY	98.5

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 416 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000155 (490 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0157 Search term: triB Search term: Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB

Human Homologs

References

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. Mima T, Joshi S, Gomez-Escalada M, Schweizer HP J Bacteriol 2007 Nov;189(21):7600-9 PubMed ID: 17720796