Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1430
|
Name |
lasR
|
Replicon | chromosome |
Genomic location | 1558171 - 1558890 (+ strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_250121.1 |
GI | 15596627 |
Affymetrix | PA1430_lasR_at |
DNASU | PaCD00006658 |
Entrez | 881789 |
GenBank | AAG04819.1 |
INSDC | AAG04819.1 |
NCBI Locus Tag | PA1430 |
protein_id(GenBank) | gb|AAG04819.1|AE004572_7|gnl|PseudoCAP|PA1430 |
TIGR | NTL03PA01431 |
UniParc | UPI000012E231 |
UniProtKB Acc | P25084 |
UniProtKB ID | LASR_PSEAE |
UniRef100 | UniRef100_P25084 |
UniRef50 | UniRef50_P25084 |
UniRef90 | UniRef90_P25084 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
transcriptional regulator LasR
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -1.68 |
Kyte-Doolittle Hydrophobicity Value | -0.020 |
Molecular Weight (kDa) | 26.6 |
Isoelectric Point (pI) | 6.51 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6MVN | TRANSCRIPTION | 10/26/18 | LasR LBD L130F:3OC10HSL complex | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2.2 | X-RAY DIFFRACTION | 99.6 |
6MWL | TRANSCRIPTION | 10/29/18 | LasR LBD:mBTL complex | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.5 | X-RAY DIFFRACTION | 100.0 |
6MWH | TRANSCRIPTION | 10/29/18 | LasR LBD:BB0020 complex | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2.2 | X-RAY DIFFRACTION | 100.0 |
6V7X | DNA BINDING PROTEIN/Viral protein | 12/09/19 | Structure of a phage-encoded quorum sensing anti-activator, Aqs1 bound to LasR | Pseudomonas aeruginosa (strain UCBPP-PA14); Pseudomonas virus DMS3 | 2.9 | X-RAY DIFFRACTION | 100.0 |
6V7W | DNA BINDING PROTEIN/Viral protein | 12/09/19 | Crystal structure of LasR-Aqs1 complex from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain UCBPP-PA14); Pseudomonas virus DMS3 | 3 | X-RAY DIFFRACTION | 100.0 |
3IX4 | TRANSCRIPTION | 09/03/09 | LasR-TP1 complex | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
3IX8 | TRANSCRIPTION | 09/03/09 | LasR-TP3 complex | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
2UV0 | TRANSCRIPTION | 03/08/07 | Structure of the P. aeruginosa LasR ligand-binding domain bound to its autoinducer | PSEUDOMONAS AERUGINOSA | 1.8 | X-RAY DIFFRACTION | 100.0 |
3IX3 | TRANSCRIPTION | 09/03/09 | LasR-OC12 HSL complex | Pseudomonas aeruginosa | 1.4 | X-RAY DIFFRACTION | 100.0 |
6MWZ | TRANSCRIPTION | 10/30/18 | LasR LBD T75V/Y93F/A127W:BB0126 | Pseudomonas aeruginosa (strain UCBPP-PA14); Pseudomonas aeruginosa UCBPP-PA14 | 1.657 | X-RAY DIFFRACTION | 98.7 |
6MWW | TRANSCRIPTION | 10/30/18 | LasR LBD:BB0126 complex | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2.76 | X-RAY DIFFRACTION | 100.0 |
3JPU | TRANSCRIPTION | 09/04/09 | LasR-TP4 complex | Pseudomonas aeruginosa | 2.3 | X-RAY DIFFRACTION | 100.0 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
N-3-OXO-DODECANOYL-L-HOMOSERINE LACTONE | DB08324 | DrugBank | 5.1.4 | P25084 | Transcriptional activator protein LasR | 100.0 | 239 | 2.3e-135 |
Source 1 | |
Database | Victors : 4258 |
Category | N-(3-oxo-dodecanoyl)-L-homoserine lactone QS system |
Evidence |
ECO:0000015
mutant phenotype evidence
|
Host Organism | Mus musculus |
Notes | |
Reference | 8557368 |
Source 2 | |
Database | VFDB : VF0093 |
Category | N-(3-oxo-dodecanoyl)-L-homoserine lactone QS system |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 142 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001096 (1439 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1430
Search term: lasR
Search term: transcriptional regulator LasR
|
Purification and characterization of LasR as a DNA-binding protein.
You Z, Fukushima J, Ishiwata T, Chang B, Kurata M, Kawamoto S, Williams P, Okuda K
FEMS Microbiol Lett 1996 Sep 1;142(2-3):301-7
PubMed ID: 8810514
|
Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression.
Gambello MJ, Iglewski BH
J Bacteriol 1991 May;173(9):3000-9
PubMed ID: 1902216
|
Intracellular receptor-type transcription factor, LasR, contains a highly conserved amphipathic region which precedes the putative helix-turn-helix DNA binding motif.
Fukushima J, Ishiwata T, Kurata M, You Z, Okuda K
Nucleic Acids Res 1994 Sep 11;22(18):3706-7
PubMed ID: 7937080
|