Pseudomonas aeruginosa PAO1, PA3544 (algE)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3544
Name	algE Synonym: alg76
Replicon	chromosome
Genomic location	3968449 - 3969921 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1

Cross-References

RefSeq	NP_252234.1
GI	15598740
Affymetrix	PA3544_algE_at
Entrez	879838
GenBank	AAG06932.1
INSDC	AAG06932.1
NCBI Locus Tag	PA3544
protein_id(GenBank)	gb|AAG06932.1|AE004775_3|gnl|PseudoCAP|PA3544
TIGR	NTL03PA03544
UniParc	UPI000012584E
UniProtKB Acc	P18895
UniProtKB ID	ALGE_PSEAE
UniRef100	UniRef100_P18895
UniRef50	UniRef50_P18895
UniRef90	UniRef90_P18895

Product

Feature Type	CDS
Coding Frame	1
Product Name	Alginate production outer membrane protein AlgE precursor Product Name Confidence: Class 1
Synonyms	alginate production protein AlgE
Evidence for Translation
Charge (pH 7)	-20.33
Kyte-Doolittle Hydrophobicity Value	-0.642
Molecular Weight (kDa)	54.4
Isoelectric Point (pI)	4.67

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Class 1 Outer Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
5IYU	MEMBRANE PROTEIN	03/24/16	AlgE_CIM	Pseudomonas aeruginosa PAO1	2.7	X-RAY DIFFRACTION	100.0
7ACG	MEMBRANE PROTEIN	09/10/20	In meso structure of the alginate exporter, AlgE, from Pseudomonas aeruginosa in 9.8 monoacylglycerol	Pseudomonas aeruginosa	1.85	X-RAY DIFFRACTION	100.0
4B61	MEMBRANE PROTEIN	08/08/12	In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1. Crystal form 3.	PSEUDOMONAS AERUGINOSA PAO1	2.402	X-RAY DIFFRACTION	100.0
4AFK	MEMBRANE PROTEIN	01/19/12	In meso structure of alginate transporter, AlgE, from Pseudomonas aeruginosa, PAO1	PSEUDOMONAS AERUGINOSA	1.897	X-RAY DIFFRACTION	100.0
5D5D	TRANSPORT PROTEIN	08/10/15	In meso in situ serial X-ray crystallography structure of AlgE at 100 K	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	2.4	X-RAY DIFFRACTION	100.0
4XNL	TRANSPORT PROTEIN	01/15/15	X-ray structure of AlgE2	Pseudomonas aeruginosa PAO1	2.9	X-RAY DIFFRACTION	100.0
4XNK	TRANSPORT PROTEIN	01/15/15	X-ray structure of AlgE1	Pseudomonas aeruginosa PAO1	2.8	X-RAY DIFFRACTION	100.0
3RBH	TRANSPORT PROTEIN	03/29/11	Structure of alginate export protein AlgE from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.301	X-RAY DIFFRACTION	100.0
4AZL	MEMBRANE PROTEIN	06/26/12	In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1, crystal form 2.	PSEUDOMONAS AERUGINOSA	2.8	X-RAY DIFFRACTION	100.0

Virulence Evidence

Source 1
Database	VFDB : VF0091
Category	Alginate biosynthesis
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 6 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002122 (490 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3544 Search term: algE Search term: Alginate production outer membrane protein AlgE precursor

Human Homologs

References

Mucoid conversion of Pseudomonas aeruginosa by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung. Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JIA, Jensen P, Johnsen AH, Givskov M, Ohman DE, Søren M, Høiby N, Kharazmi A Microbiology (Reading) 1999 Jun;145 ( Pt 6):1349-1357 PubMed ID: 10411261

Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa. Chu L, May TB, Chakrabarty AM, Misra TK Gene 1991 Oct 30;107(1):1-10 PubMed ID: 1743507