Pseudomonas aeruginosa PAO1, PA3554 (arnA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3554
Name
arnA
Synonym: arnA
Replicon chromosome
Genomic location 3982021 - 3984009 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252244.1
GI 15598750
Affymetrix PA3554_at
Entrez 878473
GenBank AAG06942.1
INSDC AAG06942.1
NCBI Locus Tag PA3554
protein_id(GenBank) gb|AAG06942.1|AE004776_5|gnl|PseudoCAP|PA3554
TIGR NTL03PA03554
UniParc UPI00000C5A65
UniProtKB Acc Q9HY63
UniProtKB ID ARNA_PSEAE
UniRef100 UniRef100_Q9HY63
UniRef50 UniRef50_O52325
UniRef90 UniRef90_Q9HY63

Product

Feature Type CDS
Coding Frame 1
Product Name
ArnA
Product Name Confidence: Class 2
Synonyms PmrI
ArnA
Evidence for Translation
Detected using LC-MS/MS (74.36 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.52
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 74.4
Isoelectric Point (pI) 6.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
arnA arnA modifies lipid A with 4-amino-4-deoxy-L-arabinose (Ara4N) which allows gram-negative bacteria to resist the antimicrobial activity of cationic antimicrobial peptides and antibiotics such as polymyxin. arnA is found in E. coli and P. aeruginosa. ARO:3002985 protein homolog model Perfect 1363.2 100.0 3.0.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 651 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002112 (439 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3554
Search term: arnA
Search term: ArnA

Human Homologs

Ensembl 97, assembly GRCh38.p12
UDP-glucuronate decarboxylase 1 [Source:HGNC Symbol;Acc:HGNC:17729]
E-value: 1.3e-27
Percent Identity: 27.3
Ensembl 97, assembly GRCh38.p12
UDP-glucuronate decarboxylase 1 [Source:HGNC Symbol;Acc:HGNC:17729]
E-value: 1.3e-27
Percent Identity: 27.3

References

PmrA-PmrB-regulated genes necessary for 4-aminoarabinose lipid A modification and polymyxin resistance.
Gunn JS, Lim KB, Krueger J, Kim K, Guo L, Hackett M, Miller SI
Mol. Microbiol. 1998 Mar;27(6):1171-82
PubMed ID: 9570402
A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose.
Breazeale SD, Ribeiro AA, McClerren AL, Raetz CR
J. Biol. Chem. 2005 Apr;280(14):14154-67
PubMed ID: 15695810
Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis.
Williams GJ, Breazeale SD, Raetz CR, Naismith JH
J. Biol. Chem. 2005 Jun;280(24):23000-8
PubMed ID: 15809294
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344