Pseudomonas aeruginosa PAO1, PA3554 (arnA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3554
Name	arnA Synonym: arnA
Replicon	chromosome
Genomic location	3982021 - 3984009 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	NP_252244.1
GI	15598750
Affymetrix	PA3554_at
Entrez	878473
GenBank	AAG06942.1
INSDC	AAG06942.1
NCBI Locus Tag	PA3554
protein_id(GenBank)	gb|AAG06942.1|AE004776_5|gnl|PseudoCAP|PA3554
TIGR	NTL03PA03554
UniParc	UPI00000C5A65
UniProtKB Acc	Q9HY63
UniProtKB ID	ARNA_PSEAE
UniRef100	UniRef100_Q9HY63
UniRef50	UniRef50_O52325
UniRef90	UniRef90_Q9HY63

Product

Feature Type	CDS
Coding Frame	1
Product Name	ArnA Product Name Confidence: Class 2
Synonyms	PmrI ArnA
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected using LC-MS/MS (74.36 kDa, PMID:21751344)
Charge (pH 7)	-3.52
Kyte-Doolittle Hydrophobicity Value	-0.253
Molecular Weight (kDa)	74.4
Isoelectric Point (pI)	6.68

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
arnA	arnA modifies lipid A with 4-amino-4-deoxy-L-arabinose (Ara4N) which allows gram-negative bacteria to resist the antimicrobial activity of cationic antimicrobial peptides and antibiotics such as polymyxin. arnA is found in E. coli and P. aeruginosa. [PMID:11021929, PMID:15939024]	ARO:3002985	protein homolog model		Perfect	1363.2	100.0	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 651 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002112 (427 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3554 Search term: arnA Search term: ArnA

Human Homologs

Ensembl 110, assembly GRCh38.p14	ENSP00000387019 UDP-glucuronate decarboxylase 1 [Source:HGNC Symbol;Acc:HGNC:17729] E-value: 1.5e-27 Percent Identity: 27.3
Ensembl 110, assembly GRCh38.p14	ENSP00000283148 UDP-glucuronate decarboxylase 1 [Source:HGNC Symbol;Acc:HGNC:17729] E-value: 1.5e-27 Percent Identity: 27.3

References

PmrA-PmrB-regulated genes necessary for 4-aminoarabinose lipid A modification and polymyxin resistance. Gunn JS, Lim KB, Krueger J, Kim K, Guo L, Hackett M, Miller SI Mol Microbiol 1998 Mar;27(6):1171-82 PubMed ID: 9570402

A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. Breazeale SD, Ribeiro AA, McClerren AL, Raetz CR J Biol Chem 2005 Apr 8;280(14):14154-67 PubMed ID: 15695810

Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis. Williams GJ, Breazeale SD, Raetz CR, Naismith JH J Biol Chem 2005 Jun 17;280(24):23000-8 PubMed ID: 15809294

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344